This pine wood nematode pwn has caused severe damage to the forest ecosystems of far east asian countries and north america. The pine sawyer beetle, a longhorned borer, spreads the nematode. The pinewood nematode, bursaphelenchus xylophilus steiner and buhrer nickle, is one of the most destructive invasive species, causing the wilting and eventual death of pine trees. Since its first diagnosis in portugal in 1999, it has been subjected to quarantine measures with impact on forest health and ecosystem stability, significantly affecting international trade of wood products. Parasitaphelenchidae article pdf available in canadian journal of forest research 3710. Bursaphelenchus xylophilus an overview sciencedirect topics.
This pathogenic nematode is believed to be native to the usa, but it was transported to several countries, including china, japan, south korea, and portugal. It invades the tracheal system of beetle species of the genus. After devastating vast areas of pine forests in asian countries, the pine wilt disease spread into european forests in 1999 and is causing worldwide concern. Nematicidal activities of three naphthoquinones against the. Bursaphelenchus xylophilus, commonly known as pine wood nematode or pine wilt nematode pwn, is a species of nematode that infects pine trees and causes the disease pine wilt. An indigenous species in north america and the causal agent of pine wilt disease, b. The pinewood nematode, bursaphelenchus xylophilus is the causative agent of pine wilt disease. Nematicidal activity of cassia and cinnamon oil compounds and.
Major sperm protein bxmsp10 is required for reproduction. Given that bursaphelenchus species are usually hard to distinguish from one. The study presents the nematicidal effects of halogenated. Molecular and biochemical characterization of an endo. Pinewood nematode, bursaphelenchus xylophilus, a pine parasitic nematode, poses a serious threat to its host pine forests globally.
Parasitaphelenchidae article pdf available in canadian journal of. Much of the information on the pathogenicity of the pwn is based. We report the cloning and functional characterization of an endo. Bursaphelenchus xylophilus is a quarantined migratory endoparasite known to cause severe economic losses in pine forest ecosystems. Here, we found pine chemical volatiles, rather than the common dauer recovery factors of. Molecular characterization of portuguese populations of. Pine mortality in japan was first reported munemoto yano. Pine wood nematode, bursaphelenchus xylophilus annual. Pine wood nematode bursaphelenchus xylophilus is a microscopic worm native to north america, and the causal agent of the pine wilt disease. Survival of bursaphelenchus xylophilus in wood chips. The japanese pine sawyer beetle as the vector of pine wilt disease. Assessments of iodoindoles and abamectin as inducers of. Buhrer nickle, is a serious forest pest, causing enormous economic losses in pine trees in korea, china, japan, and countries in western europe. This page was last edited on 12 december 2019, at 20.
The nematode feeds and multiplies in the trees resin canals, causing wilting and death in several weeks to several months. To prevent pine wilt disease pwd, trunk injection with nematicide is performed in korea. Bursaphelenchus xylophilus is the pathogen that causes pine wilt disease pwd. Apr 01, 2014 pm 74 3 bursaphelenchus xylophilus pm 74 3 bursaphelenchus xylophilus 20140401 00. Rapid diagnosis of the infection of pine tree with pine wood. Pdf first detection of bursaphelenchus xylophilus associated with.
Bursaphelenchus xylophilus, the causal agent of pine wilt disease, is a destructive threat to pine forests. Kondo e, foudin a, linit m, smith m, bolla r, winter r, dropkin v, 1982. The introduction of this nematode into japan had devastating effects on the native pines in that country. Pm 74 3 bursaphelenchus xylophilus, eppo bulletin 10. Sutherland pacifie forestry centre, natural resources canada, \ficoria, b. Bursaphelenchus xylophilus stei ner and bu h rer n ickle b.
It is only present in invertebrates and may be a suitable chemotherapeutic target in the control of this pest. Since that first report, bursaphelenchus xylophilus has been found in 36 states, including all the great plains states except for north dakota. Comparaison entre bursaphelenchus xylophilus et des especes similaires. This disease involves very complicated interactions between a pathogenic nematode, its vector beetle, host pine species, and fungi in dead hosts. Aug 19, 2019 bursaphelenchus xylophilus, the causal agent of pine wilt disease, is a destructive threat to pine forests. Distribution and reproduction of bursaphelenchus xylophilus. Vectors of this nematode are longhorned beetles especially those belonging to the genus monochamus. Bursaphelenchus is a genus of nematodes roundworms in the order aphelenchida. This is the first gene of this type from any nematode species. Major sperm protein bxmsp10 is required for reproduction and.
Bursaphelenchus mucronatus, the closest related species, differs from b. Its natural means of transmission from tree to tree is by transfer through activity of the adult stages of wood. The disease has caused significant economic losses and damage to forests. Distribution and reproduction ofbursaphelenchus xylophilus populations in wood and bark ofwestern north american conifers thomas a.
Bursaphelenchus xylophilus is a dioecious organism hasegawa and miwa, 2008. Two months after inoculation of pine wiltkilled pinus densiflora logs with e. Pm 74 3 bursaphelenchus xylophilus pm 74 3 bursaphelenchus xylophilus 20140401 00. A novel rapid sampling method for pinewood nematode, bursaphelenchus xylophilus nematoda. First report of matricidal hatching in bursaphelenchus. Assessing risks of pine wood nematode bursaphelenchus xylophilus transfer between wood packaging by simulating assembled pallets in service. Identification of potential nematicidal compounds against the. Author summary bursaphelenchus xylophilus is an important plant pathogen, responsible for an epidemic of pine wilt disease in asia and europe. Text is available under the creative commons attributionsharealike license. Based on dna sequences of mitochondrial cytochrome oxidase subunit i mtcoi genes, three pcr forward primers specific for bursaphelenchus mucronatus mucronatus, b. Jul 28, 2017 bursaphelenchus xylophilus is a quarantined migratory endoparasite known to cause severe economic losses in pine forest ecosystems. Multifaceted approach for differentiating isolates of.
The pinewood nematode pwn bursaphelenchus xylophilus, the causal agent of the pine wilt disease pwd, enters aboveground parts of the tree, migrates through the resin canals and feeds on plant cells causing extensive damage. Comparison of bursaphelenchus xylophilus with similar species. It also occurs in japan, china, taiwan, korea, and portugal history. Pdf assessing risks of pine wood nematode bursaphelenchus. Jul 18, 2012 since bursaphelenchus xylophilus causes serious losses in pine forestry, new ways of controlling this nematode damage are urgently needed.
Bursaphelenchus xylophilus, the causative agent of pine wilt disease, has been associated with heavy losses of native japanese. Bursaphelenchus xylophilus, the pine wood nematode, is an eppo a2 pest. Identification of potential nematicidal compounds against. Molecular characterization of portuguese populations of the. Cloning and characterization of a 2cys peroxiredoxin in.
Pm 91 6 bursaphelenchus xylophilus and its vectors. General information about bursaphelenchus xylophilus bursxy eppo global database. Biological control of the pinewood nematode bursaphelenchus. We show that a similar cdna is also present in another closely related species b. Rapid diagnosis of the infection of pine tree with pine. Many bursaphelenchus species are obligate fungus feeders, while b.
Bursaphelenchus xylophilus, pine wood nematode host and distribution pine wood nematode b. The pine wood nematode, bursaphelenchus xylophilus, is an invasive plant parasitic nematode and a worldwide quarantine pest. The pinewood nematode, bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Dec 12, 2016 the pinewood nematode, bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi. The antiphytoalexin gene bxcathepsin w supports the. Bursaphelenchus xylophilus may infest trees without. Cloning arginine kinase gene and its rnai in bursaphelenchus. Nematicidal activity of cassia and cinnamon oil compounds.
Bursaphelenchus xylophilus was first described in, and is perhaps native to, north america. It also occurs in japan, china, taiwan, korea, and portugal. Pdf a novel rapid sampling method for pinewood nematode. Bursaphelenchus xylophilus may infest trees without expression of wilt. Although these nematicidal agents are quite efficient, the development of new nematicidal agents is needed to prevent. Since its first report in japan in 1905, pwn has become an economically important pest throughout the east asian. Esteya vermicola ophiostomataceae is the first reported endoparasitic fungus of the pinewood nematode pwn, bursaphelenchus xylophilus nematoda. Detection of pinewood nematode, bursaphelenchus xylophilus, using a realtime polymerase chain reaction assay.
Detection of the pinewood nematode, bursaphelenchus xylophilus. Dec 31, 2019 bursaphelenchus xylophilus was first described in, and is perhaps native to, north america. Pathogenicity of the pine wood nematode is determined not only by its physical and chemical. Pinewood nematodes 157 bursaphelenchus mucronatus has also been found in many asian and european countries 36,39,45,46,52,62.
Aphelenchoididae, and one common pcr reverse primer were developed to determine the interspecific hybrids in a population. Bursaphelenchus xylophilus is the causal agent of pine wilt disease and a worldwide pest with high economic impact. Details about its biology, distribution and economic importance can be found in eppocabi 1997. Observation and quantification of mating behavior in the. At the beginning of the 20th century, the disease was especially prevalent in east asian countries.
Bursaphelenchus wikispecies wikispecies, free species. The pine wood nematode, bursaphelenchus xylophilus steiner and buhrer, 1934 nickle 1970, is the causal agent of pine wilt disease. Results were compared with those of 34 related compounds. In order to penetrate the cell wall and establish a parasitic relationship with host trees, the pwn needs to secretea mixture of active cell wall degrading enzymes. The diagnostic procedure for bursaphelenchus xylophilus is presented in fig. Pm100228 eppo a1 and a2 lists of pests recommended for regulation as quarantine pests 2019.
The pine wood nematode bursaphelenchus xylophilus, the causative agent of a devastating epidemic of pine wilt disease, is a migratory endoparasite that causes severe damage to pine forests in east asia and europe 1, 2. Introduction 44 the pinewood nematode pwn, bursaphelenchus xylophilus, is a migratory plant. Cystatins are involved in various biological processes where they regulate normal proteolysis and also play a role in pathogenicity, but their functions in b. Documents about bursaphelenchus xylophilus bursxy number title download. Most are obligate mycophages, but some feed on wood, with two species, the red ring nematode b. Pine chemical volatiles promote dauer recovery of a pine. Arginine kinase ak is a phosphotransferase, which plays a critical role in cellular energy metabolism in invertebrates. Nematicidal activities of three naphthoquinones against. The pinewood nematode, bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi. Pine wood nematode is a quarantine harmful organism in the eu where its spread would cause. Bursaphelenchus xylophilus, the pine wood nematode, is an eppo a1 pest. A simple method for distinguishing bursaphelenchus. Attraction of the bursaphelenchus xylophilus nematode toward 18 volatiles of pinus species was evaluated by a petridish bioassay under laboratory conditions to develop a rapid diagnostic kit. Media in category bursaphelenchus xylophilus the following 33 files are in this category, out of 33 total.
The pine wood nematode bursaphelenchus xylophilus is a migratory endoparasitic nematode causing pine wilt disease pwd. Pm 74 3 bursaphelenchus xylophilus 20 eppo bulletin. The nematode is native to north america canada, usa, mexico unconfirmed and is thought to have been carried to japan at the beginning of the 20th century on timber exports. Bursaphelenchus xylophilus an overview sciencedirect. It occurs in much of the united states, canada, and mexico. In both the nematodes native habitat in north america and in new epidemic areas in east asia and europe, b.
Oct 16, 2012 bursaphelenchus xylophilus is the causal agent of pine wilt disease and a worldwide pest with high economic impact. Genomic insights into the origin of parasitism in the. Since bursaphelenchus xylophilus causes serious losses in pine forestry, new ways of controlling this nematode damage are urgently needed. Specific approval and amendment first approved in 2002. Specific scope this standard describes a diagnostic protocol for bursaphelenchus xylophilus. The book details the proceedings of an international workshop held at the university of evora in 2001, covering all major aspects of the bioecology of the pinewood nematode pwn, bursaphelenchus xylophilus, its insect vectors and effects on the tree.